Composite

Part:BBa_K4941103:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


ku70UP-ylER-EcYida-URA-ku70dw


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6663
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1553
    Illegal XhoI site found at 1951
    Illegal XhoI site found at 5080
    Illegal XhoI site found at 5113
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6663
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6663
    Illegal NgoMIV site found at 5268
    Illegal AgeI site found at 2430
    Illegal AgeI site found at 4210
    Illegal AgeI site found at 6038
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1399
    Illegal BsaI.rc site found at 814
    Illegal BsaI.rc site found at 2753
    Illegal BsaI.rc site found at 4533
    Illegal SapI site found at 1701
    Illegal SapI.rc site found at 522


Design Notes

none



Source

yarrowia lipolytica

References